Hélène Guilmette graduated in piano and musical education in Quebec's Laval University and studied voice with Marlena Malas in New York. In 2004 she was awarded Second Prize at Queen Elisabeth Competition.

On the operatic stage, she was Sophie (Werther) in Brussels and Lille, Valencienne (The Merry Widow) at the Opéra Comique, Pedro (Don Quichotte) at Tokyo New National Theater, Frasquita (Carmen) in Avignon, Servilia (Clemenza di Tito) and Crobyle (Thaïs) in Montreal. In 2007-2008 she made her debut in Brussels as Pamina (Die Zauberflöte) and as Sophie (Werther) and also at the Paris Opera as Amor (Orpheo and Eurydice) and as Mélisande (Ariane et Barbe Bleue). Recently, she sang Susanna (Le Nozze di Figaro) in Lille and at the Théâtre des Champs-Elysées, Sophie (Werther) in Strasbourg, Sœur Constance (Dialogues des Carmélites) in Munich and Nice, Mélisande (Ariane et Barbe Bleue) in Amsterdam's Concertgebouw and Thérèse (Les Mamelles de Tirésias) in Lyon and Paris.

Alongside of Andreas Scholl, with whom she recorded Handel's Duello Amoroso in 2007, she performed in Schwarzenberg's Shubertiade in Austria, in Istanbul Music festival, as well as in Quebec with Les Violons du Roy and at the Théâtre des Champs-Elysées for Pergolesi's Stabat Mater. Her discography includes Airs chantés (2005), french mélodies by Poulenc, Hahn and Daunais, Handel's Dixit Dominus and Ode for the Anniversary of Queen Anne (2009) with the Akademie für Alte Musik Berlin.

Her projects include Dialogues des Carmélites (sœur Constance) in Toronto's COC, Amadis de Gaule by Johann Christian Bach at the Opéra Comique, Orphée et Eurydice (Eurydice) at Anger-Nantes Opera, Le Nozze di Figaro (Susanna) and Falstaff (Nanetta) in Montreal Opera, Les Indes galantes (Hébé) by Rameau in Toulouse and Werther (Sophie) at the Paris National Opera.

Hélène Guilmette received support of the Canada Council of the art, the Conseil des arts et lettres du Québec and the Fondation Jacqueline Desmarais.